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e9h80  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc e9h80
    E9h80, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v5+tag/V5-Tag+Mouse+mAb/pm42805951-315-26-27
    Average 95 stars, based on 72 article reviews
    e9h80 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Mechanistic insights into CAR-mediated repression of the HNF4α-FABP1 axis and inhibition of HepG2 cell proliferation.
    Article Snippet: .. Subsequently, membranes were blocked with 5% milk and incubated with antibodies against CAR (diluted 1:1000; PP-N4111–00, Perseus Proteomics), FABP1 (diluted 1:1000; sc− 271591, Santa Cruz Biotechnology), V5-Tag (diluted 1:1000; #13202 s, Cell Signaling Technology), HNF4α (diluted 1:1000; sc− 374229, Santa Cruz Biotechnology), HNF4α P1 (diluted 1:1000; PPK9218–00, Perseus Proteomics), HNF4α P2 (diluted 1:1000; PPH6939–00, Perseus Proteomics), Acetyl-Histone H3 diluted (Lys9/ Lys14) (diluted 1:1000; #9677 s, Cell Signaling Technology) Histone H3 (diluted 1:1000, #4620, Cell Signaling Technology) or β-actin (diluted 1:5000; A3854, Sigma-Aldrich) at 4◦C overnight. .. Blots were washed and incubated with horseradish peroxidase secondary antibodies and developed with West Pico chemiluminescent substrates (ThermoFisher).

    Article Title: The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma
    Article Snippet: DT-061 (HY-112929) and DT-766/TRC-766 (HY-131443) were purchased from MedChem Express, OA was purchased from P212121, and cycloheximide solution was purchased from Sigma-Aldrich (C4859). .. Membranes were blocked in 5% non-fat milk/TBST for 1 hour and incubated overnight at 4 °C with primary antibodies: c-Myc (ABclonal, A19032, 1:1000), phospho S62 c-Myc (Abcam, ab185656, 1:1000), N-Myc (Abcam, ab16898, 1:1000), Vinculin (Santa Cruz Biotech, sc-73614, 1:5,000), GAPDH (Santa Cruz Biotech, sc-32233, 1:2000), V5-tag (Cell Signaling Technology, 13,202, 1:1000), AP2β (Cell Signaling Technology, 2509, 1:1000), GATA3 (Cell Signaling Technology, 5852, 1:1,000), PHOX2B (Santa Cruz Biotech, sc-376997, 1:1,000), PARP (Cell Signaling Technology, 9542L, 1:1000), Cleaved caspase-3 (Cell Signaling Technology, 9664L, 1:1,000), and PPP2R2A (Santa Cruz Biotech, sc-81606, 1:1000). .. Whole-cell lysates were extracted by RIPA (Thermo Fisher Scientific, PI89901) supplemented with protease (Roche, 05892791001) and phosphatase (Roche, 4906837001) inhibitors.

    Article Title: The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma.
    Article Snippet: PageRuler Prestained Protein Ladder (Fisher Scientific, PI26617) was used to confirm expected kDa of targets (Fisher Scientific, PI26617). .. Membranes were blocked in 5% non-fat milk/TBST for 1 h and incubated overnight at 4°C with primary antibodies: c‐Myc (ABclonal, A19032, 1:1000), phospho S62 c-Myc (Abcam, ab185656, 1:1000), N‐Myc (Abcam, ab16898, 1:1000), Vinculin (Santa Cruz Biotech, sc‐73614, 1:5000), GAPDH (Santa Cruz Biotech, sc‐32233, 1:2,000), V5-tag (Cell Signaling Technology, 13202, 1:1000), AP-2 beta (Cell Signaling Technology, 2509, 1:1000), GATA3 (Cell Signaling Technology, 5852, 1:1000), HAND2 (Santa Cruz Biotech, sc-398167, 1:1000), PHOX2B (Santa Cruz Biotech, sc-376997, 1:1000), PARP (Cell Signaling Technology, 9542L, 1:1000), Cleaved caspase‐3 (Cell Signaling Technology, 9664L, 1:1000), and PPP2R2A (Santa Cruz Biotech, sc-81606, 1:1000). ..

    FLAG-tag:

    Article Title: Targeting kinesin family member 20A sensitizes stem-like triple-negative breast cancer cells to standard chemotherapy
    Article Snippet: Signals were visualized using enhanced chemiluminescence (ECL; Millipore, WBULS0100) and imaged with a ChemiDoc Imaging System (Bio-Rad) using Image Lab software. .. Primary antibodies used were as follows: KIF20A (1:1,000; Proteintech, 15911-1-AP), SMARCA4/BRG1 (1:1,000; Cell Signaling Technology, 49360S), histone H3 (1:2,000; Cell Signaling Technology, 4499T), ARID1A (1:100; Santa Cruz Biotechnology, SC32761), MVB12B (1:200; Sigma-Aldrich, HPA043683), V5 tag (1:1,000; Cell Signaling Technology, 13202S), HA tag (1:1,000; Cell Signaling Technology, 3724S), FLAG tag (1:1,000; Cell Signaling Technology, 14793S), vinculin (1:5,000; Sigma-Aldrich, V9131), α-tubulin (1:1,000; Cell Signaling Technology, 3873S), and β-actin (1:1,000; Cell Signaling Technology, 3700S). .. Total RNA was extracted with RNeasy Mini Kit (QIAGEN, 74104) following the manufacturer’s protocol.

    other:

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth
    Article Snippet: Antibodies used: TSSK6 (sc-514076, Santa Cruz) (1:500 IB), STAT3 (sc-8019, Santa Cruz (1:1000 IB), STAT3 (9139, CST)(1:1000 IB, 1:300 IF), pSTAT3 (Ser727) (9134, CST)(1:1000 IB), pSTAT3 (Y705) (9131, CST), Vinculin (sc-73614, Santa Cruz) (1:2000 IB), Beta-Tubulin (2128, CST) (1:5000), V5-tag (anti-rabbit,13202, CST) (1:5000 IB), V5-tag (anti-mouse, 46-0705, Invitrogen)(1:2000 IB), PLEKHA7 (PA5-85686, Invitrogen) (1:1000 IB), MEF2C (ab227085, Abcam)(1:5000 IB), pFAK (Y397) (44-624G, Invitrogen) (1:1000 IB, 1:200 IF), FAK (3286, CST)(1:1000 IB).

    Western Blot:

    Article Title: Loss of EHMT2 enhances NK cell-driven anti-tumor immunity through TGF-β1 suppression
    Article Snippet: β-Actin (D6A8) (Diluted at 1:2000 for WB) , Cell Signaling Technology , 8457 RRID: AB_10950489. .. V5-Tag (Diluted at 1:1000 for WB) , Cell Signaling Technology , 13202 RRID: AB_2687461. .. Anti-Mouse Asialo-GM1 (Injected 100 μg/mouse, once a week) , Biolegend , 146002 RRID: AB_2562206.

    Cell-Signaling:

    Article Title: The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma.
    Article Snippet: PageRuler Prestained Protein Ladder (Fisher Scientific, PI26617) was used to confirm expected kDa of targets (Fisher Scientific, PI26617). .. Membranes were blocked in 5% non-fat milk/TBST for 1 h and incubated overnight at 4°C with primary antibodies: c‐Myc (ABclonal, A19032, 1:1000), phospho S62 c-Myc (Abcam, ab185656, 1:1000), N‐Myc (Abcam, ab16898, 1:1000), Vinculin (Santa Cruz Biotech, sc‐73614, 1:5000), GAPDH (Santa Cruz Biotech, sc‐32233, 1:2,000), V5-tag (Cell Signaling Technology, 13202, 1:1000), AP-2 beta (Cell Signaling Technology, 2509, 1:1000), GATA3 (Cell Signaling Technology, 5852, 1:1000), HAND2 (Santa Cruz Biotech, sc-398167, 1:1000), PHOX2B (Santa Cruz Biotech, sc-376997, 1:1000), PARP (Cell Signaling Technology, 9542L, 1:1000), Cleaved caspase‐3 (Cell Signaling Technology, 9664L, 1:1000), and PPP2R2A (Santa Cruz Biotech, sc-81606, 1:1000). ..



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